WebRRM2 is upregulated in many cancer types, and its suppression is proposed to result in cell cycle arrest and cell death. CALAA-01 entered a phase 1 clinical trial and treatment was continued until the disease progressed or the treatment could no longer be tolerated by the patient. 21% of patients discontinued the trial due to adverse effects. WebOct 30, 2014 · Set1 employs its RNA-binding RRM2 and catalytic SET domains to repress Tf2 retrotransposons and pericentromeric repeats while relying on its H3K4me function to maintain transcriptional repression at the silent mating …
RRM2 - Wikipedia
WebJul 1, 2024 · Combinatorial RRM2-CHK1 inhibition acts synergistic in high-risk … WebOct 17, 2024 · The RRM2 subunit is regulated by two pathways: the SETD2-catalysed H3K36me3 mark promotes RRM2 expression; and WEE1 inhibits the activity of cyclin-dependent kinases CDK1 and CDK2 through tyrosine ... insulin is what type of hormone
Targeting the USP7/RRM2 axis drives senescence and sensitizes …
DLD1 cells were transfected with Flag-MYBL2 or Flag-TAF15 for 48 h, then cross-linked with 1% formaldehyde and sonicated DNA to ~200 bp. The supernatants obtained by centrifugation was immunoprecipitated overnight by the antibody against FLAG or normal rabbit IgG at 4 °C. Chromatin–protein-antibody … See more Antibodies against RRM2(sc-398294), RRM2B(sc-10840), LIN9(sc-398234), and Cyclin E(sc-247) were from Santa Cruz Biotech (Santa Cruz, CA). The MYBL2(ab12296), … See more The human CRC cell lines DLD1, HCT116, and SW480 were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and … See more The fresh paired cancer and para-cancer normal tissues, and formalin-fixed, paraffin-embedded (FFPE) cancer tissues of clinical CRC patients were obtained from the Second … See more HCT116 cell layers on glass coverslips were fixed for 15 min by 4% paraformaldehyde at 4 °C. Then, we used PBS containing 0.2% Triton X-100 permeabilizing cells for 20 min and blocking with PBS … See more WebFeb 15, 2010 · Here, we found that ribonucleotide reductase (RNR) subunits RRM1 and … WebChromatin immunoprecipitation (ChIP) Chromatin was immunoprecipitated from 3×106 A498 cells crosslinked with 1 mM Bis-(sulfosuccinimidyl) glutarate and 1% formalde-hyde according to the protocol [22] with some modifications. Briefly, isolated chromatin was broken into 100-500 bp fragments by sonica-tion (Bioruptor UCD-200) for five cycles (the insulin is usually administered