Recombinase cre activity
Webb12 apr. 2024 · PDF The Cre/lox recombination system has become a powerful technology for gene function analysis in a broad spectrum of cell types and organisms. In... Find, read and cite all the research you ... WebbExcision of c-Jun with Cre recombinase, in c-junf1/f1 3T3 cells, abrogated DACH1-mediated inhibition of DNA synthesis. c-Jun expression rescued DACH1-mediated inhibition of cellular proliferation. DACH1 inhibited induction of c-Jun by physiological stimuli and repressed c-jun target genes (cyclin A, β-PAK, and stathmin).
Recombinase cre activity
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Webb12 apr. 2024 · Firefly luciferase activity was determined by the luminescence measurement of catalyzed D-luciferin in electroporated BY-2-xGxFL cells using different poring pulse conditions: field strength (50, 100, 150, 200, or 250 V/cm), duration (10 or 20 ms). In all cases, 5 μM Cre protein dissolved in Opti-MEMI was used for electroporation. Webbactivity after tamoxifen induction. Recombinase activity was confined to sensory neurons and no expression was found in other organs. Less than 1% of neurons showed Cre expression in the absence of tamoxifen treatment. Five-day intraperitoneal treatment with tamoxifen (2 mg per day) induced Cre recombination events in ≈90% of
Webb批注本地保存成功，开通会员云端永久保存 去开通 WebbNotes: Black boxes indicate sites of Cre activity detected in the original report of each Cre line (ref. in last column) and references in each black/grey box refer to additional manuscripts reporting Cre activity.An empty box indicates Cre activity has not been reported (it does not exclude possible Cre activity). Asterisks denote knock-in lines. …
WebbThe site-directed recombinase Cre can be employed to delete or express genes in cell lines or animals. Clearly, the ability to control remotely the activity of this enzyme would be highly desirable. WebbThe IRES-Cre-cDNA fragment was inserted into a 173-kb bacterial artificial chromosome (BAC) carrying the intact Pcp2 gene, by using red-mediated recombineering. Transgenic mice were generated with the modified BAC and identified. The Cre-transgenic mice were crossed with ROSA26 and Z/EG reporter mice to detect Cre-recombinase activity. …
Webb1 feb. 2024 · These types of animal models have been valuable for many studies but their limitations can get in the way of some experiments. A conditional knockout allele can achieve the same result, ... positioned in a head-to-head orientation to enable inversion by Cre recombinase inserted into the anti-sense strand of a target gene.
WebbCancer-associated fibroblasts (CAFs) are present in all malignancies. Arguably, in none are they as prevalent as they are in pancreatic ductal adenocarcinoma (PDAC), where they often outnumber cancer cells. The origin and function of CAFs are still not completely understood, and attempts to target this cell population as a component of combination … heller v district of columbia ap gov defWebbFör 1 dag sedan · Gaglia et al. find striking changes in the spatial arrangement of immune cells in response to tumor antigens. T and B cells are recruited in lymphocyte networks (“lymphonets”), which contain progenitor T cells. After immunotherapy, lymphonets gain cytotoxic T cells, likely due to progenitor cell differentiation and activation in this distinct … heller urban dictionaryWebbThe CreER recombinase is inactive but can be activated by the synthetic estrogen receptor ligand tamoxifen or 4-hydroxytamoxifen. The more improved versions of the chimeric … hellerup staircaseWebb1 apr. 1996 · Several fusions between Cre recombinase and the hormone binding domain (HBD) of a mutated human progesterone receptor, which binds RU 486 but not progesterone, were constructed. When tested in transient expression assays recombination activities of all fusion proteins were responsive to RU 486, but not to the … heller\u0027s williamsport pa menuWebb17 sep. 2024 · Tamoxifen (TAM) inducible Cre recombinase system is an essential tool to study gene function when early ablation or overexpression can cause developmental … lakemont memorial gardens find a graveWebb17 sep. 2024 · The inducible Cre/loxP estrogen receptor (ER) transgenic system is used to study both ubiquitous and tissue-specific gene function through the expression of Cre … heller v chicagoWebbDNA recombinase enzymes such as Cre or FLP recombinases detect specific flanking sequences that, depending on their orientation, allow for excision or rotation of the … lakemont mobile home park acworth ga