Primer efficiency range
WebThe amplification efficiency (E) for each target can be calculated according to the following equation: E = 10 (–1/S) – 1 (where S = slope of the standard curve) To compare the … WebFor an efficiency of 100%, the slope is -3.32. A good reaction should have an efficiency between 90% and 110%, which corresponds to a slope between -3.58 and -3.10. R2 Value …
Primer efficiency range
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WebHow to use the calculator. 1. Calculate the slope of the curve during the primer efficiency qPCR. If you are struggling to determine the slope of the curve, check out our guide on …
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