WebPhosphoamidase — In enzymology, a phosphoamidase (EC number 3.9.1.1) is an enzyme that catalyzes the chemical reaction:N phosphocreatine + H2O ightleftharpoons creatine … Phosphoramidates (sometimes also called amidophosphates) are a class of phosphorus compounds structurally related to phosphates (or organophosphates) via the substitution of an OR for a NR2. They are derivatives of phosphoramidic acids O=P(OH)(NR2)2, O=P(OH)2(NR2). A phosphorodiamidate (or diamidophosphate) is a phosphate that has two of its OH groups substituted by NR2 groups to give a species with the general formula O=P(OH)(NH2)2. The subs…

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WebAug 1, 1999 · A 13-kDa phosphoamidase was isolated as a single band on SDS-PAGE from bovine liver. Its Stokes' radius, sedimentation coefficient, molecular mass, and optimal pH … WebSUMMARY Phosphoamidase, an enzyme that catalyzes the hydrolysis of amides of phosphoric acid, has been purified about 50-fold from an extract of beef spleen. Enzymic … elizabeth stickers

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WebThe histochemical technique used to demonstrate Phosphoamidase activity (E.C. 3.9.1. 1) was a modification of the standard alkaline phosphatase technique. Root segments, 2-4 mm long, were fixed in 6 per cent glutaraldehyde for 30 min and washed in 0-005 m tris maleate buffer, pH 7-2, with three changes for 30 min. They were then incubated in a WebA 13-kDa phosphoamidase was isolated as a single band on SDS-PAGE from bovine liver. Its Stokes' radius, sedimentation coefficient, molecular mass, and optimal pH were … WebAlkaline phosphatase is a homodimeric protein enzyme (86 kDa), containing two zinc atoms crucial to its catalytic function per monomer, and it is optimally active at alkaline pH environments. The enzyme is found in prokaryotes and eukaryotes, with the same general function but in different structural forms suitable to organism's environment. forcep tubing clamp