Web1 mrt. 2002 · Methylphosphonate oligonucleotides are noncharged oligomers, in which a nonbridging oxygen atom is replaced by a methyl group at each phosphorus in the oligonucleotide chain. Although these oligonucleotides have excellent stability in biological systems ( 10 ), the absence of charge reduces their solubility. WebPhosphonocarboxylate oligonucleotides are recently-developed modifications that can be incorporated using “phosphoramidite-like” monomers. 1,2 The monomers, shown in Figure 1 are formally called “phosphonamidites or phosphinocarboxylic acid amidites” because they contain a carbon atom attached to the phosphorus atom instead of a protected oxygen.
Design and applications of modified oligonucleotides - SciELO
WebOligonucleotide-based therapies are a promising approach for treating a wide range of hard-to-treat diseases, particularly genetic and rare diseases. These therapies involve the use of short synthetic sequences of DNA or RNA that can modulate gene expression or inhibit proteins through various mechanisms. Despite the potential of these therapies, a … Web6 feb. 2003 · Oligo-2‘-O-methylribonucleotides conjugated with 4-(2-aminooxyethoxy)-2-(ethylureido)quinoline (AOQ) and 4-ethoxy-2-(ethylureido)quinoline (EOQ) were prepared by reaction of the AOQ or EOQ phosphoramidite with the protected oligonucleotide on a controlled pore glass support. pure-hearted definition
Antisense Oligonucleotides: Basic Concepts and Mechanisms
WebHistorically, methylphosphonate oligonucleotide synthesis has been inefficient, costly, and difficult to conduct above a l-µmol scale. We have devised methods that significantly reduce cost, increase coupling efficiency, improve recovery, and are applicable to larger-scale synthesis. Webneutral analogs, methylphosphonate oligo-mers (Figure 2-1a) and phosphoramidates (Figure 2-1b) have been readily prepared on solid supports. Although these analogs have an increased resistance to nucleases, they show a lower RNA binding affinity than natural phosphodiester-based parent se-quences. This reduced affinity has been at-DNA mRNA ... WebAdditionally, the methylphosphonate linkage is resistant to nuclease hydrolysis. Here we show that a single methylphosphonate internucleotide linkage at the 3'-end of an oligo-2'-O-methylribonucleotide is sufficient to prevent degradation by the 3'-exonuclease activity found in mammalian serum. pure heart church in glendale az