Bulked segregant analysis bsa dna sequencing
WebApr 14, 2024 · Bulked segregant RNA-seq (BSR-seq) couples BSA with RNA-seq (Liu et al., 2012) and can be used not only to identify differentially expressed genes (DEGs) but also single-nucleotide polymorphisms (SNPs) that are tightly linked to targeted traits (Du et al., 2024). BSR-seq has been successfully applied in analyses of many species. WebApr 13, 2024 · Here, bulked segregant RNA-Seq was used to identify two candidate genes for melanoid, which, in comparison to the wild type, is characterized by increased …
Bulked segregant analysis bsa dna sequencing
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WebTwo bulks with 30 plants each were further selected from the segregating population for sequencing along with the parental lines. High throughput sequencing data was used for BSA. We identified 10 QTLs on Chr 1, Chr 2, Chr 3, … WebApr 11, 2024 · A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with SoySNP50K BeadChips, revealing that the deletion on Chr 16 might be responsible for the altered phenotype. ... followed by a bulked segregant analysis (BSA) using whole genome …
Background. Bulked segregant analysis (BSA), coupled with next-generation sequencing, allows the rapid identification of both qualitative and quantitative trait loci (QTL), and this technique is referred to as BSA-Seq here. The current SNP index method and G-statistic method for BSA-Seq data analysis … See more In BSA-Seq studies, if a SNP is not associated with the trait, its REF/ALT reads would be randomly segregated in both bulks, and the … See more The sSNPs should cluster around the genes controlling the trait phenotype on the chromosomes due to linkage disequilibrium. Using the sliding window technique, the number of sSNPs was plotted across all the … See more The results in Figs. 1b and 2 indicated that the significant SNP method had higher detection power. However, different methods were used … See more Using the Lander/Waterman equation [23], the sequencing coverage of SRR834927 and SRR834931 was estimated to be 84× and 103×, respectively. It would be very costly to achieve … See more WebPericarp color in wheat is an important trait related to nutrients and health benefits. Identification of molecular markers closely linked to the grain color g
WebBulked-segregant analysis is a strategy of genetic mapping by constructing two bulks of individuals with contrasting phenotypes from the segregating population. Bulked-segregant analysis sequencing (BSA-seq), based on next-generation sequencing technology, is an effective tool in mapping genetic loci underlying agronomic traits (especially for ... WebJan 20, 2024 · The BSA-NGS analysis revealed that two candidate loci from 1.7 to 2.0 Mbp and 5.6 to 6.2 Mbp on chromosome (Chr) 9 were associated with dwarfism. We validated these SNPs in F2 progeny using a high-resolution melting assay. Based on the results, we identified a perfect cosegregation region of 1,872,994 to 1,866,655 bp on Chr 9.
WebDNA extraction, library construction, and sequencing. Allele frequency estimates in BSA analysis depend on the variation in segregant samples and sequencing technology. …
WebAug 31, 2024 · Most mitochondrial electron transport chain (mtETC) complex genes were upregulated in fertile compared to sterile buds. Using bulked segregant analysis (BSA)-seq to analyze mixed DNA pools from sterile and fertile F 2 buds, respectively, we identified a 6.25 Mb candidate interval where Rfw is located. datamatics global services inc in arizonaWebIn this study, the Cry2Ab resistance gene in T. ni was mapped to Chromosome 17 by genetic linkage analyses using a whole genome resequencing approach, and was then finely mapped using RNA-seq-based bulked segregant analysis (BSA) and amplicon sequencing (AmpSeq)-based fine linkage mapping to a locus containing two genes, … martini srl piedimonteWebApr 11, 2024 · A bi-parental population derived from the mutant G15FN-54 and the cultivar Benning was developed to conduct a bulked segregant analysis (BSA) with … datamatics global services ltd linkedinWebJan 28, 2024 · Key message Using bulked segregant analysis of exome sequence, we fine-mapped the ABA-hypersensitive mutant ERA8 in a wheat backcross population to … datamatics global services puneWebBulk segregation analysis (BSA) utilizes a strategy of pooling individuals with extreme phenotypes to conduct economical and rapidly linked marker screening or quantitative … martini stefanoWebBulked segregant analysis ( BSA) is a technique used to identify genetic markers associated with a mutant phenotype. This allows geneticists to discover genes conferring … datamatics global share price nseWebQTL mapping is a fundamental approach for understanding the genetic inheritance of DM resistance in cucumber. Recently, many studies have reported that a combination of … martini store.it